FAS ELISA Kit, Human

BACKGROUND Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis.¹ The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40.² The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa.³ Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance.⁴ Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.⁵ The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.⁶ The standard product used in this kit is FAS extracellular sections (1-173aa), in addition to the soluble human IgG1 Fc domain. The molecular mass is 45KDa.

 

REFERENCES  

1.Dhein, J, et al: Nature 373: 438-441, 1995.
2.Itoh, N.; et al: Cell 66: 233-243, 1991.
3.Oehm, A.; et al: J. Biol. Chem. 267: 10709-10715, 1992.
4.Arscott, P. L. et al: J. Clin. Endocr. Metab. 84: 4246-4252, 1999.
5.Desbarats, J.; et al: Nature Cell Biol. 5: 118-125, 2003.
6.Zhang, X. et al: J. Med. Genet. 42: 479-484, 2005.

 

 

ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.