IL-21: Mouse Interleukin-21 | sputnic-group.ru : Cell Applications

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IL-21: Mouse Interleukin-21

BACKGROUND Interleukin-21 (IL-21) is a member of the common gamma-chain family of cytokines (includes IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15) and is expressed by activated CD4+ T cells. IL-21 has a private receptor known as IL-21R, which is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple Ab classes in vivo. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells.

Recombinant mouse IL-21 is a non-glycosylated protein, containing 130 amino acids and having a total molecular mass of 15.1 kDa.

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Параметры

Cat. No.:

RP2070

Alternative Name:

None

Source:

E. coli

Physical Appearance:

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation:

Recombinant mouse IL-21 was lyophilized from 20 mM NaHCO3, pH 8.5.

Stability:

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.

Reconstitution:

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Protein Content and Purity determined by:

UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.

Endotoxin Level:

Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.

Biological Activity:

The activity is determined by a dose-dependent proliferation assay using BaF3 cells and is typically 10-50 ng/mL.

AA Sequence:

MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

Документы

Product Sheet RP2070

IL-21

Название	Код	Цена
Mouse Interleukin-21	RP2070-1000	по запросу
Mouse Interleukin-21	RP2070-10	по запросу
Mouse Interleukin-21	RP2070-100	по запросу

Факторы роста и Цитокины

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