QUANTIFOIL™ is a holey carbon film with a thickness of about 12 nm, that is applied on a standard copper or gold electron microscopy grid and provides an ideal support for biological samples in Cryo-EM techniques[1-6].
The supporting mesh (copper or gold) is available with distinct numbers. Higher mesh numbers (400) indicate closely spaced bars and provide higher stability, whereas lower numbers (200) provide larger free faces.
The QUANTIFOIL™ carbon film has circular holes of defined size and interspace, e.g. R 2/1 (pictured) has holes of 2 µm diameter with an interspace of 1 µm in both dimensions. Higher magnifications usually require smaller holes and vice versa.