Нейробластома человека - клеточная линия 2xT25 культуральные колбы Растущая культура
Organism: Homo sapiens (human) Age: 4 years old Gender: Female Tissue: Brain (from metastatic site: bone marrow) Morphology: The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float; a confluent monolayer is not formed. Cell type: Neuroblast (neuroblastoma) Growth Properties: Loosely adherent; form clumps at high cell density Description: SH-SY5Y is one of three serially isolated neuroblast clones (SH-SY, SH-SY5, SH-SY5Y) of the human neuroblastoma cell line SK-N-SH which was established in 1970 from a metastatic bone tumor. The cells exhibit moderate levels of dopamine beta hydroxylase activity. They can convert glutamate to the neurotransmitter GABA. SH-SY5Y cells have a reported saturation density greater than 1 x 106 cells/cm2. The loss of neuronal characteristics has been described with increasing passage numbers (approx. passage 20). Neuronal markers or uptake of noradrenalin should be determined routinely. It is recommended not to use at passage numbers higher than 20 unless neuronal characteristics are controlled. Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой |
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