Каталог > Клеточные линии, штаммы, среды > Культуральные среды > Культуральные среды с лизатом тромбоцитов человека (hPL) >
DMEM:F12 medium kit hPL DMEM:F12 medium kit hPL


DMEM:Hams F12 среда - набор
250 мл среды, базовой
12.5 ml hPL supplement (5% final), deep-frozen

DMEM:Ham’s F12 cell culture medium supplemented with hPL (human Platelate lysate)

The DMEM:Ham’s F12 is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham’s F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less. Furthermore, it is an excellent basic medium for the serum free cell culture.

The supplement hPL (human Platelet lysate) is manufactured from platelet single donor apheresis samples which are collected at blood banks in Southern Germany. Contrary to FBS, hPL is a bystander product. Platelet preparations must be destroyed 5 days after collection from the donors unless they are used up in other applications. Only platelets from double-negative tested donors are used to manufacture the platelet lysates and are pooled from 25 donors.

DMEM:Ham’s F12 supplemented with hPL delivers sufficient nutrients and growth factors to sustain the proliferation of cell lines such as A-549 lung cancer cells and NCI-H295R adrenal gland carcinoma cells.

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