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РНК тканей

Tissue RNA

Total RNA prepared from normal human tissues using the modified guanidium thiocyanate method.  RNA is DNAse treated to remove contaminating genomic DNA and undergoes stringent quality control tests.  Purified RNA is ready for use in convenient 50 µg and 250 µg sizes for various downstream applications, including microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.  Stringent quality control tests confirm RNA integrity and stability.

  • Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37C  incubation
  • No visible Genomic DNA band of 23 kb detected
  • No genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primers
  • A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers
  • A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis
  • Primers were designed to detect mRNA for genes of interest to confirm cell phenotype

 

Публикации

2015
Yoshida, K., H. Aburatani and S. Ishikawa. 2015. ANTI-DLL3 ANTIBODY. Patent Application US 20150368355 A1.
2014
Eikmans, M., J. Anholts, and F. Claas. 2014. Gene Expression Analysis by qPCR in Clinical Kidney Transplantation. Methods in Molec Biol, 1160:147-163.
2012
Al Menhali, A. 2012. Parathyroid Hormone-Like Hormone (PTHLH): A Novel Parietal Cell Growth Factor Regulated by Gastrin. PhD Dissertation, U Michigan.
Mok, P., S. Cheong, C. Leong, K. Chua, and O. Ainoon. 2012. Extended and stable gene expression via nucleofection of MIDGE construct into adult human marrow mesenchymal stromal cells. Cytotechnology, 64:203-216.
Soroida, Y., R. Ohkawa, H. Nakagawa, Y. Satoh, H. Yoshida, H. Kinoshita, R. Tateishi, R. Masuzaki, K. Enooku, S. Shiina, T. Sato, S. Obi, T. Hoshino, R. Nagatomo, S. Okubo, H. Yokota, K. Koike, Y. Yatomi, and H. Ikeda. 2012. Increased activity of serum mitochondrial isoenzyme of creatine kinase in hepatocellular carcinoma patients predominantly with recurrence. J Hepatology, 57:330-336.
Tanabe, Y. Y. Fujiware, A. Matsuzaki, E. Fujita, T. Kasahara, S. Yuasa, and T. Momoi. 2012. Temporal expression and mitochondrial localization of a Foxp2 isoform lacking the forkhead domain in developing Purkinje cells. J Neurochm, 122:72-80.  
2011
Yoshida, K., H. Aburatani, and S. Ishikawa. 2011. Anti-dll3 antibody. Patent Application US 20120328624 A1.
2010
Sogawa, C., C. Mitsuhata, K. Kumagai-Morioka, N. Sogawa, K. Ohyama, K. Morita, K. Kozai, T. Dohi, and S. Kitayama. 2010. Expression and Function of Variants of Human Catecholamine Transporters Lacking the Fifth Transmembrane Region Encoded by Exon 6. PLOS One, DOI: 10.1371/journal.pone.0011945.
2009
Aburatani, H., S. Ishikawa, and S. Kawai. 2009. Diagnosis and treatment of cancer using anti-tm4sf20 antibody. Patent Application US 20120004117 A1.
Aburatani, H., S. Tsutsumi, K. Nishimura, H. Sakumoto, and S. Kawai. 2009. Anti-cldn6 antibody. Patent US 20110059469 A1.


НазваниеКодЦена
Total RNA prepared from human brain tissue, fetal 1F01-50 по запросу
Total RNA prepared from human brain tissue, fetal 1F01-250 по запросу



НазваниеКодЦена
Total RNA prepared from human heart tissue 1H30-50 по запросу
Total RNA prepared from human heart tissue 1H30-250 по запросу



НазваниеКодЦена
Total RNA prepared from human small intestine tissue 1H24-50 по запросу
Total RNA prepared from human small intestine tissue 1H24-250 по запросу



НазваниеКодЦена
Total RNA prepared from human kidney tissue 1H50-50 по запросу
Total RNA prepared from human kidney tissue 1H50-250 по запросу



НазваниеКодЦена
Total RNA prepared from human liver tissue 1H21-50 по запросу
Total RNA prepared from human liver tissue 1H21-250 по запросу
Total RNA prepared from human liver tissue, fetal 1F21-50 по запросу
Total RNA prepared from human liver tissue, fetal 1F21-250 по запросу



НазваниеКодЦена
Total RNA prepared from human lung tissue 1H40-50 по запросу
Total RNA prepared from human lung tissue 1H40-250 по запросу



НазваниеКодЦена
Total RNA prepared from human pancreas tissue 1H23-50 по запросу
Total RNA prepared from human pancreas tissue 1H23-250 по запросу



НазваниеКодЦена
Total RNA prepared from human placental tissue 1H54-50 по запросу
Total RNA prepared from human placental tissue 1H54-250 по запросу



НазваниеКодЦена
Total RNA prepared from human prostate tissue 1H52-50 по запросу
Total RNA prepared from human prostate tissue 1H52-250 по запросу



НазваниеКодЦена
Total RNA prepared from human skeletal muscle tissue 1H60-50 по запросу
Total RNA prepared from human skeletal muscle tissue 1H60-250 по запросу



НазваниеКодЦена
Total RNA prepared from human spleen tissue 1H70-50 по запросу
Total RNA prepared from human spleen tissue 1H70-250 по запросу



НазваниеКодЦена
Total RNA prepared from human stomach tissue 1H20-50 по запросу
Total RNA prepared from human stomach tissue 1H20-250 по запросу



НазваниеКодЦена
Total RNA prepared from human testes tissue 1H51-50 по запросу
Total RNA prepared from human testes tissue 1H51-250 по запросу



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