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Anti-HCV E2: Rabbit HCV E2 Antibody

BACKGROUND The hepatitis C virus (HCV) particle consists of a core of genetic material (RNA), surrounded by an icosahedral protective shell of protein, and further encased in a lipid (fatty) envelope of cellular origin. Two viral envelope glycoproteins, E1 and E2, are embedded in the lipid envelope (1). The proteins of this virus are arranged along the genome in the following order: N terminal-core-envelope (E1)–E2–p7-nonstructural protein 2 (NS2)–NS3–NS4A–NS4B–NS5A–NS5B–C terminal. The mature nonstructural proteins (NS2 to NS5B) generation relies on the activity of viral proteinases (2). Both envelope proteins (E1 and E2) are highly glycosylated and important in cell entry. E1 serves as the fusogenic subunit and E2 acts as the receptor binding protein. E1 has 4–5 N-linked glycans and E2 has 11 N-glycosylation sites (3). E1 and E2 glycoproteins form a heterodimer that is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some additional liver specific cofactors may be needed. The fusion function may possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.





1. Op De Beeck A, et al. (2003) Rev. Med. Virol.13, 233–41.
2. De Francesco R, et al. (1999) J Hepatol.31, 47–53.
3. Grakoui A, et al. (1993) J Virol.67, 1385-1395.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.


Antigen:Mammalian cell expressed recombinant protein of HCV E2
Isotype:Rabbit IgG
Species & predicted
species cross-
reactivity ( ):
Applications &
Suggested starting
WB         1:1600-1:1800
IP                    n/d
IHC                 n/d
ICC                 n/d
FACS               n/d
Predicted Molecular
Weight of protein:
36~41, 58~70kDa
Specificity/Sensitivity:Detects endogenous HCV E2 proteins without cross-reactivity with other family members.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.

Rabbit HCV E2 Antibody CC1059 по запросу

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