1. Каталог >
  2. Клеточные линии, штаммы, среды >
  3. Клеточная биология >
  4. Антитела >

Anti-TFIIB: Monoclonal Transcription Factor II B Antibody (48C6)

BACKGROUND Transcription Factor II B (TF IIB) is an essential component of the RNA polymerase II (Pol II) machinery that acts as a bridge between the TATA-binding protein (TBP) and Pol II.1 The C-terminal domain of TFIIB interacts with the TBP-TATA complex, whereas the N-terminal domain is required for the assembly of TFIIF and Pol II into the preinitiation complex. It is involved in the selection of the transcription start site: mutations in TFIIB cause a shift in the transcription start site.2 In addition, TFIIB interacts with certain TBP-associated factors (TAFs) in vitro, and these interactions might contribute to recruitment or stable association of TFIIB in the context of an active transcription complex.3
1. Ha, I. et al: Nature 352:689, 1991.
2. Lee, T. I. & Young,  R. A. : Annu. Rev. Genet. 34:77, 2000.
3. Goodrich, J. A. et al: Cell 75:519, 1993.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.


E. coli-expressed recombinant human TFIIB protein fragments.
Mouse monoclonal IgG2b
Species & predicted
species cross-
reactivity ( ):
Applications &
Suggested starting
WB                  1:1000
IP                    n/d
IHC (Paraffin)  n/d
ICC                 n/d
FACS              n/d
Predicted Molecular
Weight of protein:
38 kDa
Specifically detects endogenous levels of human TFIIB proteins. Does not cross-react with other related proteins.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

 *Optimal working dilutions must be determined by end user.


Monoclonal Transcription Factor II B Antibody (48C6) CB14574 по запросу

Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой