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Anti-Cyclin D2: Polyclonal Cyclin D2 Antibody

BACKGROUND Cyclin-D2 is encoded by the CCND2 gene in human. It belongs to the highly conserved cyclin family that control cell cycle progression in an orderly fashion from G1 to S- to G2/M.1 In mammalian cells, progression through the cell cycle is governed by a family of Cdks, whose activity is regulated by phosphorylation, activated by binding of cyclins, and inhibited by Cdk inhibitors. Three D-type cyclins (D1, D2, and D3) are expressed in G1 phase of the cell cycle and depending on cell lineage. D-type cyclins form complexes with and activate cdk2, -4, and -6 duringthe G1 phase of the cell cycle.2 The INK4 family of cell cycle inhibitors (P16INK4a, P15INK4b, P18INK4c, and P19INK4d) negatively regulates the activity of Cdk4/6 by preventing cyclin D binding.  A key substrate for G1 cyclin/cdk complexes is the retino-blastoma protein, pRb. Phosphorylation of pRb, a tumor suppressor gene product, has been attributed to cyclin/cdk complexes and implicated in the regulation of proliferation in mammalian cells, phosphorylation of pRb blocks its ability to suppress the activity of S phase promoting transcription factors such as E2F. Apart from the positive roles that D-type cyclins play in cell cycle progression, a number of recent studies have shown that D-type cyclins also perform cdk-independent functions and, in certain cell types, even prevent cdks from being activated.3 Up-regulation of cyclin D2 has been implicated in human gastric carcinoma.4
1. Tsihlias J et al.: Annu. Rev. Med., 50: 401–423, 1999.
2. Sweeney KJ et al.: Oncogene 14:1329 - 1340, 1997.
3. Pagano M et al.: Genes Dev., 8, 1627 - 1639, 1994.
4. Nakayama OY et al.: Int J Oncol.;23:1663-70, 2003.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.


Peptide containing human Cyclin D2 N-terminal sequence.
Rabbit Polyclonal
Species & predicted
species cross-
reactivity ( ):
Human, Rat, Mouse
Applications &
Suggested starting
WB                  1:1000
IP                    1:50
IHC (Paraffin)  1:100
ICC                  n/d
FACS               n/d
Predicted Molecular
Weight of protein:
30 kDa
Detects endogenous levels of Cyclin D2 protein in  normal primary cell lysates.
Store at 4° C for frequent use; at -20° C for at least one year.

*Optimal working dilutions must be determined by end user.


Polyclonal Cyclin D2 Antibody CA1225 по запросу

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