Legionella pneumophila |
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Legionaminic acid (LEG) is an essential lipopolysaccharide component exclusively synthesized by Legionella pneumophila.
The Azide-functionalized Legionaminic acid precursor pLEG-Azide (Man2NAc4NAc6-N3) provides an attractive approach for the specific detection of Legionella pneumophila in living cultures: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart.
The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of (Desthio)Biotin or DBCO-containing (Desthio)Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of Fluorescent Dyes or DBCO-containing Fluorescent Dyes , respectively) for subsequent microscopic imaging.
Tested Legionella pneumophila strains[1]:
Legionella pneumophila SG1 (Paris), Legionella pneumophila SG1 (Lens), Legionella pneumophila SG1 (Philadelphia), Legionella pneumophila SG3, Legionella pneumophila SG4, Legionella pneumophila SG5, Legionella pneumophila SG6
[1] Mas Pons et al. (2014) Identification of Living Legionella pneumophila using species-specific metabolic lipopolysaccharide Labeling. Angew. Chem. Int. Ed. 53:1275.
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