Mycobacteria |
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Myobacteria are characterized by a trehalose-lipopolysaccharide-containing outer cell membrane.
The Azide-functionalized 6-Azido-Trehalose provides an attractive approach for the detection of Myobacteria in living cultures[1]: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart.
The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of (Desthio)Biotin or DBCO-containing (Desthio)Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of Fluorescent Dyes or DBCO-containing Fluorescent Dyes , respectively) for subsequent microscopic imaging.
Myobacteria strains tested[1]:
Mycobacterium smegmatis mc2155, Mycobacterium tuberculosis H37Rv, Mycobacterium bovis BCG
[1] Swarts et al. (2012) Probing the Myobacterial Trehalome with Bioorthogonal Chemistry. J. Am. Chem. Soc. 134(39):16123.
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