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Клетки эндотелия капилляров кожи человека: CADMEC/HMVEC. Первичные клеточные линии Cell Applications.

Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC

Клетки Эндотелия Капилляров Кожи (CADMEC/HMVEC) от Cell Applications, Inc. обеспечивают прекрасную модельную систему для изучения многих аспектов эндотелиальных функций и заболеваний, особенно связанных с микрососудистой и капиллярной системами. Эти клетки были торговой маркой Cell Applications, Inc. более 20 лет назад, когда они были впервые предложены, и CADMEC™ означало “Cell Applications’ Dermal Microvascular Endothelial Cells”. Было показано, что HMVEC/CADMEC экспрессируют vWF, CD36 и CD31, способны поглощать DiI-Ac-LDL и являются положительными на функциональную активность при цитокин-стимулированной адгезии лейкоцитов.

Избранные лоты HMVEC были дополнительно тестированы для демонстрации стимуляционно-зависимого ангиогенеза и ключевых для эндотелиальных клеток сигнальных путей (фосфорилирование VEGFR, Akt, MAPK, и экспрессию Tie2, eNOS, Axl и Etk/Bmx). Более подробную информацию смотрите в разделе Тестированные Эндотелиальные Клетки.

CADMEC/HMVEC от Cell Applications, Inc. были использованы во многих исследованиях, например:

(Текст на языке оригинала)
  • Show that cytokine-activated microvascular endothelial cells upregulate expression of α2-6-linked sialic acids which are the ligands for CD22 on the surface of the B cells, increasing the adhesion between the two cell types
  • Show that IL-1, TNF and IFN stimulated ICAM-1 and E-selectin production by microvascular endothelial cells and resulted in a higher leukocyte adhesion that could be prevented by dexamethasone
  • Demonstrate that FGF-2, but not FGF-13, induces proliferation of microvascular endothelial cells, and that neither growth factor affected IL-6 production
  • Map VEGF signaling pathway by demonstrating that c-Src phosphorylates VEGFR-2 and IQGAP1, leading to activation of b-Raf, causing endotheial cell proliferation and angiogenesis
  • Demonstrate that mechanical signals promote endothelial cell proliferation via VEGFR2/Akt signaling cascade leading to inactivation of GSK3β and preventing cyclin D1 degradation
  • Show that endothelial permeability caused by advanced glycation end products results from RhoA/ROCK and p38 dependent moesin phosphorylation leading to actin reorganization
  • Demonstrate, along with Human Coronary Artery Endothelial Cells, also от Cell Applications, Inc., the role of AMP-activated protein kinase (AMPK) in preservation of the tight junctions, explaining the heightened vascular permeability when AMPK is inhibited due to exposure to lipopolysaccharides during sepsis
  • Demonstrate that the endothelial to mesenchymal transition associated with portal venous stenosis is mediated by increased serum levels of TGF-b1 in idiopathic portal hypertension patients, which induced fibroblast-like morphology, reduction of CD34 expression, and induction of α-smooth muscle actin, COL1A1 and phospho-Smad2, and that BMP-7 was able to prevent these changes (Kitao, 2009);
  • Reveal a critical role for the α7 nAChR in mediating the effects of nicotine on the endothelium
  • Show that abnormal matrix composition characteristic for systemic sclerosis, leads to reduced proliferation, reduced NO-to-O2- ratio, increased apoptosis, and altered protein expression associated with endothelial to mesenchymal transition, all leading to impaired vascular function and angiogenesis
  • Develop anti-tumor apoptosis-inducing peptides that selectively target angiogenic cells and a targeted gene delivery system, PEI-g-PEG-RGD, for efficient expression of soluble fragment of VEGF receptor Flt-1 (sFlt-1) gene in order to inhibit tumor-related angiogenesis
  • Identify a novel tumor-derived angiogenic factor, gastrin-releasing peptide, and its small molecule inhibitor, 77427
  • Evaluate, along with Human Pulmonary Artery Endothelial Cells (HPAEC) and Human Umbilical Cord Vascular Endothelial Cells (HUVEC), all obtained от Cell Applications, Inc., the cytotoxic effects of a novel pore-forming protein, proposed as an anti-tumor agent
  • To confirm the antioxidant and anti-inflammatory effects of blueberry and cranberry anthocyanins and hydroxycinnamic acids against H2O2 and TNFα induced damage to microvascular endothelial cells, by demonstrating reduction in oxidative stress and lowered production of IL-8, MCP-1 and ICAM-1 and show that procyanidins isolated from cocoa inhibit the expression of ErbB2 gene, decreasing cell proliferation and angiogenesis (Kenny, 2004);
  • Demonstrate secretion of adipogenic factor(s) by microvascular endothelial cells


Normal human neonatal foreskin or adult skin capillaries.  Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi. 
Cryopreserved ampoule:
2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Each kit contains an ampoule of cryopreserved CADMEC (100-05), 500 ml of CADMEC Cell Growth Medium (112-500), a Subculture Reagent Kit, two Extracellular Matrix Attachment Factor Coated T-25 Flasks (121-25-02), 100 ml of Attachment Factor Solution (123-100).
Proliferating Cells:
Shipped in Endothelial Cell Transfer Media at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings




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Harris, L.J., P. Zhang, H. Abdollahi, N.A. Tarola, C. DiMatteo, S.E. McIlhenny, T.N. Tulenko, and P.J. DiMuzio. 2010. Availability of Adipose-Derived Stem Cells in Patients Undergoing Vascular Surgical Procedures. J. Surgical Research. 163:e105-e112.
Liu, J., and S. Agarwal. 2010. Mechanical signals activate vascular endothelial growth factor receptor-2 to upregulate endothelial cell proliferation during inflammation. The Journal of Immunology. 185:1215-1221.
Fischer, L.J., S. McIlhenny, T. Tulenko, N. Golesorkhi, P. Zhang, R. Larson, J. Lombardi, I. Shapiro, and P.J. DiMuzio. 2009. Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. Journal of Surgical Research. 152:157-166.
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Kitao, A., Y. Sato, S. Sawada-Kitamura, K. Harada, M. Sasaki, H. Morikawa, S. Shiomi, M. Honda, O. Matsui, and Y. Nakanuma. 2009. Endothelial to mesenchymal transition via transforming growth factor-β1/Smad activation is associated with portal venous stenosis in idiopathic portal hypertension. The American journal of pathology. 175:616-626.
Wu, J.C., A. Chruscinski, V.A. De Jesus Perez, H. Singh, M. Pitsiouni, M. Rabinovitch, P.J. Utz, and J.P. Cooke. 2009. Cholinergic modulation of angiogenesis: role of the 7 nicotinic acetylcholine receptor. Journal of cellular biochemistry. 108:433-446.
Cuttittta, F., A. Martinez, and W. Stetler-Stevenson. 2008. Compositions and methods for promoting angiogenesis. Patent US 7462593 B2.
Hu, J., C. Rosen, and L. Cao. 2008. Vascular endothelial growth factor 2. Patent US 7439333 B2.
Meyer, R.D., D.B. Sacks, and N. Rahimi. 2008. IQGAP1-dependent signaling pathway regulates endothelial cell proliferation and angiogenesis. PloS one. 3:e3848.
Coman, T. Comprises nucleotide sequences associated with expression vector coding endothelial growth factor for use in diagnosis and treatment of tumor angiogenesis, inflammation, diabetic retinopathy, arthritis and psoriasis; wound healing agent. Patent US 7273751 B2.
Hu, J., C. Rosen, and L. Cao. 2007. Methods of stimulating angiogenesis in a patient by administering vascular endothelial growth factor 2. Patent US 7186688 B1.
Igarashi, K., I. Sakimoto, K. Kataoka, K. Ohta, and M. Miura. 2007. Radiation-induced senescence-like phenotype in proliferating and plateau-phase vascular endothelial cells. Experimental cell research. 313:3326-3336.
Fujiuchi, S., Y. Yamazaki, Y. Fujita, Y. Nishigaki, A. Taked, Y. Yamamoto, T. Fijikane, T. Shimizu, S. Osanai, T. Takahashi, and K. Kikuchi. 2006. S-Nitrosoglutathione (SNOG) Accumulates Hypoxia Inducible Factor-1α in Main Pulmonary Artery Endothelial Cells but not in Micro Pulmonary Vessel Endothelial Cells. In THE ARTERIAL CHEMORECEPTORS. Vol. 580. Y. Hayashida, C. Gonzalez, and H. Kondo, editors. Springer US. 63-71.
Hu, J., C. Rosen, and L. Cao. 2006. Polypeptide for use in the treatment of inflammation, rheumatoid arritis, psoriasis, and diabetic retinopathy. Patent US 7153942 B2.
Li, H., M. Adams, V. Calenda, and V. Fataccioli. 2006. Stimulating angiogenesis enhancing the repair of connective and support tissue, promoting the attachment, fixation and stabilization of tissue implants and enhancing wound healing. Patent US 7026299 B2.
Hu, C. Rosen, and L. Cao. 2005. Administering anti-Flt4 antibody or an anti-VEGF-C antibody that inhibits binding between Flt4 ligand protein and Flt4 expressed in cells to treat breast cancer characterized by endothelial cells that express Flt4 tyrosine kinase. Patent Application US 20060025331 A1.
Hu, J., C. Rosen, and L. Cao. 2005. Produced by recombinant techniques and polynucleotides encoding the polypeptides; used to stimulate angiogenesis for wound healing and for vascular tissue repair. Patent Application US 20050176103 A1.
Ji, H., P.A. Abshire, M. Urdaneta, and E. Smela. 2005. CMOS contact imager for monitoring cultured cells. In Circuits and Systems, 2005. ISCAS 2005. IEEE International Symposium on. IEEE. 3491-3494.
Kim, W.J., J.W. Yockman, M. Lee, J.H. Jeong, Y.-H. Kim, and S.W. Kim. 2005. Soluble Flt-1 gene delivery using PEI-g-PEG-RGD conjugate for anti-angiogenesis. Journal of controlled release. 106:224-234.
Li, H., M. Adams, V. Calenda, and V. Fataccioli. 2005. Connective tissue growth factor-2. Patent Application US 20060052328 A1.
Martinez, A., E. Zudaire, M. Julian, T.W. Moody, and F. Cuttitta. 2005. Gastrin-releasing peptide (GRP) induces angiogenesis and the specific GRP blocker 77427 inhibits tumor growth in vitro and in vivo. Oncogene. 24:4106-4113.
Hu, J., L. Cao, and A. Rosen. 20045. Nucleotide sequences coding polypeptide for use in the diagnosis, prevention and treatment of cancer, inflammation, diabetic retinopathy, rheumatoid arthritis and psoriasis. Patent US 6734285 B2.
Kenny, T.P., C.L. Keen, P. Jones, H.-J. Kung, H.H. Schmitz, and M.E. Gershwin. 2004. Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H2O2. Experimental biology and medicine. 229:765-771.
Duan, D., and S. Rosen. 2003. Bone marrow specific protein. Patent Application US 20030191062 A1.
Ellerby, H.M., S. Lee, L.M. Ellerby, S. Chen, T. Kiyota, G. del Rio, G. Sugihara, Y. Sun, D.E. Bredesen, W. Arap, and R. Pasqualini. 2003. An Artificially Designed Pore-forming Protein with Anti-tumor Effects. Journal of Biological Chemistry. 278:35311-35316.
Hu, J., L. Cao, and C. Rosen. 2003. Comprises nucleotide sequences coding mitogens for treating psoriasis, vision defects, tumors and arthritis. Patent Application US 20040143103 A1.
Li, H., M. Adams, V. Calenda, and V. Fataccioli. 2001. Connective tissue growth factor-2. Patent Application US 20030012768 A1.
Coleman, T. 2001. Vascular endothelial growth factor-2. Patent Application US 20030215921 A1.
Hutley, L.J., A.C. Herington, W. Shurety, C. Cheung, D.A. Vesey, D.P. Cameron, and J.B. Prins. 2001. Human adipose tissue endothelial cells promote preadipocyte proliferation. American Journal of Physiology-Endocrinology And Metabolism. 281:E1037-E1044.
Patel, N.A., J.A. Patel, M.F. Stins, K.S. Kim, and S.L. Chang. 2001. Dexamethasone Affects Cytokine-Mediated Adhesion of HL-60 Human Promyelocytic Leukemia Cells to Cultured Dermal Microvascular Endothelial Cells. Clinical Immunology. 99:387-394.
Ruoslahti, E., R. Pasqualini, W. Arap, B. Dale, and E. Michael. 2001. Chimeric prostate-homing peptide comprising a sequence of serine-methionine-serine-isoleucine-alanine-arganine-leucine, or functionally equivalent sequence. Patent Application US 20010046498 A1.
Hu, J., C. Rosen, and L. Cao. 2000. Amino acid sequence; for angiogenesis inhibitors, antitumor agents, antiinflammatory agents, antiarthritic agents for rheumatoid arthritis, wound healing agents; treatment of diabetic retinopathy and psoriasis. Patent US 6040157 A.
Leung, K.H., V. Pippalla, A. Kreutter, and M. Chandler. 1998. Functional effects of FGF-13 on human lung fibroblasts, dermal microvascular endothelial cells, and aortic smooth muscle cells. Biochem. & Biophys. Research Communic. 250:137-142.
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Polyclonal Vascular Endothelial Growth Factor Antibody CA1080 по запросу

Polyclonal VEGF Receptor 1 Antibody CB3839 по запросу

Basal medium (contains no growth supplement).В  Add GS before use. 110-500 по запросу
Basal medium without phenol red 110PR-500 по запросу

Basal medium & growth supplement sold together packaged separately 112K-500 по запросу
Growth Medium Kit without phenol red 112KPR-500 по запросу

For general cryopreservation of most primary cells. Contains FBS & DMSO. 040-50 по запросу

CADMEC Total Kit: Media, Subculture Reagents & Cells, Adult 100K-05a по запросу
CADMEC Total Kit: Media, Subculture Reagents & Cells, Neonatal 100K-05n по запросу

CADMEC Total Kit: Media, Subculture Reagents & Cells, Pre-Screened, Adult S100K-05a по запросу
CADMEC Total Kit: Media, Subculture Reagents & Cells, Pre-Screened, Neonatal S100K-05n по запросу

Cryopreserved CADMEC 100-05a по запросу

Cryopreserved CADMEC/HMVEC S100-05a по запросу

Cryopreserved Ampoule 100-05n по запросу

Cryopreserved CADMEC S100-05n по запросу

25 x 24-Well Rxns TF101KS по запросу
250 x 24-Well Rxns TF101K по запросу

Proliferating CADMEC 101-25a по запросу
Proliferating CADMEC 101-75a по запросу
Proliferating CADMEC 101-6Wa по запросу
Proliferating CADMEC 101-96Wa по запросу

Proliferating CADMEC S101-96wa по запросу
Proliferating CADMEC S101-75a по запросу
Proliferating CADMEC S101-6wa по запросу
Proliferating CADMEC S101-25a по запросу

Proliferating CADMEC 101-96Wn по запросу
Proliferating CADMEC 101-25n по запросу
Proliferating CADMEC 101-75n по запросу
Proliferating CADMEC 101-6Wn по запросу

100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K по запросу

Extended Family Products

AFS 123-100 по запросу

Total RNA prepared from Human Dermal Microvascular Endothelial Cells, adult 100-R10a по запросу
Total RNA prepared from Human Dermal Microvascular Endothelial Cells, adult 100-R25a по запросу
Total RNA prepared from Human Dermal Microvascular Endothelial Cells, Stimulated, adult 100S-R10a по запросу
Total RNA prepared from Human Dermal Microvascular Endothelial Cells, Stimulated, adult 100S-R25a по запросу
Total RNA prepared from Human Dermal Microvascular Endothelial Cells, Stimulated, neonatal 100S-R10n по запросу
Total RNA prepared from Human Dermal Microvscular Endothelial Cells, neonatal 100-R10n по запросу
Total RNA prepared from Human Dermal Microvscular Endothelial Cells, neonatal 100-R25n по запросу

100 tests 028-S по запросу
500 tests 028-01 по запросу

Human Vascular Endothelial Growth Factor-121 RP1116-10 по запросу
Human Vascular Endothelial Growth Factor-121 RP1116-100 по запросу
Human Vascular Endothelial Growth Factor-121 RP1116-1000 по запросу

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Информация представлена исключительно в ознакомительных целях и ни при каких условиях не является публичной офертой