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Клетки эндотелия аорты крысы: RAOEC. Первичные клеточные линии Cell Applications.

Rat Aortic Endothelial Cells: RAOEC

Клетки Эндотелия Аорты Крысы  (RAOEC) обеспечивают прекрасную модельную систему для изучения всех аспектов сердечно-сосудистых функций и заболеваний и были использованы в десятках научных публикаций. Положительный клеточный тест на DiI-Ac-LDL поглощение.


Adult rat aorta.  Each lot is tested negative for mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
3rd passage, >500,000 cells in Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved  RAOEC (R304-05a), 500 ml of Rat Endothelial Cell Growth Medium (R211-500), 100 ml of Attachment Factor Solution (123-100) and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Rat Endothelial Cell Growth Medium at 4th passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least to 6th passage



Iba, T., T. Hamakubo, I. Nagaoka, K. Sato and T. Jecko. 2016. Physiological levels of pentraxin 3 and albumin attenuate vascular endothelial cell damage induced by histone H3 in vitro. Microcirculation, DOI: 10.1111/micc.12269.
Otto, S., A. Deussen, B. Zatschler, B. Muller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2016.  A novel role of endothelium in activation of latent pro-membrane type 1 MMP and pro-MMP-2 in rat aorta. Cardiovascular Research, 109:409-418.
Ding, Q., Y. Hussain, J. Chorazyczewski, R. Gros, and R. Feldman. 2015. GPER-independent effects of estrogen in rat aortic vascular endothelial cells. Molecular and Cellular Endocrinology, 399:60-68.
Iaconetti, C., S. De Rosa, A. Polimeni, S. Sorrentino, C. Gareri, A. Carino, J. Sabatino, A. Curcio, and C. Indolfi. 2015. Down-Regulation of miR-23b Induces Phenotypic Switching of Vascular Smooth Muscle Cells in vitro and in vivo. Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv141.
Otto, S., A. Deussen, B. Zatschler, B.Müller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2015. A Novel Role of Endothelium in Activation of Latent Pro-Membrane Type 1 Matrix Metalloproteinase and Pro-Matrix Metalloproteinase-2 in Rat Aorta.  Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv256.
Zhu, S., M. Zhang, J. Davis, W. Wu, K. Surrao, H. Wang and G. Wu. 2015. A single mutation in helix 8 enhances the angiotensin II type 1a receptor transport and signaling. Cellular Signalling, 27:2371-2379.
Ahmed, S., N. Anzures, Z. Bosley, B. Bui, A. Feizi, S. Jawahery, C. Koenig, K. Lakomy, M. Lin, P. Natarajan, E. Nathan, H. Sayed, and E. Solano. 2014. THE APPLICATION OF AN EXOGENOUS LINEAR AND RADIAL ELECTRICAL FIELD TO AN IN VITRO CHRONIC DIABETIC ULCER MODEL FOR EVALUATION AS A POTENTIAL TREATMENT.  U Maryland, Digital Repository, http://hdl.handle.net/1903/15531.
Byun, E., N. Sung, M. Yang, D. Song, E. Byun, J. Kim, J. Park, B. Song, J. Lee, S. Park, M. Byun, and J. Kim. 2014. Procyanidin C1 Causes Vasorelaxation Through Activation of the Endothelial NO/cGMP Pathway in Thoracic Aortic Rings. Journal of Medicinal Food, 17:742-748.
Hamid, H., A. Azlina, M. Khamis, R. Rahman, N. Razak, S. Mutum, and A. Samsudin. 2014. Effect of Biphasic Calcium Phosphate Treated with Vascular Endothelial Growth Factor on Osteogenesis and Angiogenesis Gene Expression In Vitro. IFMBE Proceedings, 43:239-242.
Kopaliani, I. M. Martin, B. Zatschler, K. Bortlik, B. Müller, and A. Deussen. 2014. Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta. Basic Research in Cardiology, June, 109:419.
Mears, B., F. Shiao, N. Toney, C. Chen, K. Choi, C. Chow, M. Navin, and H. Shiau. 2014. Salvianolic Acid B Inhibits Growth of Cervical Cancer Cells in vitro via Induction of Apoptosis through the Extrinsic Pathway. U Maryland, Digital Repository.
Gros, R., Q. Ding, B. Liu, J. Chorazyczewski, and R.D. Feldman. 2013. Aldosterone mediates its rapid effects in vascular endothelial cells through GPER activation. American Journal of Physiology-Cell Physiology. 304:C532-C540.
Nguyen, H., V.L. Chiasson, P. Chatterjee, S.E. Kopriva, K.J. Young, and B.M. Mitchell. 2013. Interleukin-17 causes Rho-kinase-mediated endothelial dysfunction and hypertension. Cardiovascular Research. 97:696-704.
Padilla, J., and N. Jenkins. 2013. Induction of endoplasmic reticulum stress impairs insulin-stimulated vasomotor relaxation in rat aortic rings: role of endothelin-1. J Physiol Pharmacol. 64:557-564.
Byun, E., T. Ishikawa, A. Suyama, M. Kono, S. Nakashima, T. Kanda, T. Miyamoto, and T. Matsui. 2012. A procyanidin trimer, C1, promotes NO production in rat aortic endothelial cells via both hyperpolarization and PI3K/Akt pathways.  European Journal of Pharmacology, 692: Issues 1–3, 5 October:52–60.
Byun, E., S. Korematsu, T. Ishikawa, T. Nishizuka, S. Ohshima, T.l Kanda, and T. Matsui. 2012. Apple procyanidins induce hyperpolarization of rat aorta endothelial cells via activation of K+ channels. J Nutional Biochem, 3:278-286.
Liu, B. 2012. The role of GRK2 in hypertension and regulation of GPR30. MS Thesis, University of Western Ontario.
Liu, X., Y. Cheng, J. Yang, L. Xu and C. Zhang. 2012. Cell-specific effects of miR-221/222 in vessels: molecular mechanism and therapeutic application. J Mol Cell Cardiol. 52:245-255. RAOSMC
Masuda, Y., Y. Yamada, and Y. Kimura. 2012. In Vitro Guidance of Dental Pulp Cells by Nd:YAG Laser-Irradiated Endothelial Cells. Photomed Laser Surg, 30:315-319.
Aslam, M., D. Gündüz, D. Schuler, L. Li, F. Sharifpanah, D. Sedding, H.M. Piper, and T. Noll. 2011. Intermedin induces loss of coronary microvascular endothelial barrier via derangement of actin cytoskeleton. Cardiovascular research. 92:276-286.
Chiasson, V.L., M.A. Quinn, K.J. Young, and B.M. Mitchell. 2011. Protein Kinase CβII-Mediated Phosphorylation of Endothelial Nitric Oxide Synthase Threonine 495 Mediates the Endothelial Dysfunction Induced by FK506. J. Pharm.&Exp.Therap.337:718-723.
Duffy, G.P., T.M. McFadden, E.M. Byrne, S.-L. Gill, E. Farrell, and F.J. O’Brien. 2011. Towards in vitro vascularisation of collagen-GAG scaffolds. European cells & materials. 21:15-30.
Ibrahim, S., Q. Kang, and A. Ramamurthi. 2010. The Impact of HA Oligomer Content on Physical, Mechanical, and Biologic Properties of Divinyl Sulfone-Crosslinked HA Hydrogels. J Biomed Mater Res A, 94:355-370.
Imamura, H., T. Ohta, K. Tsunetoshi, K. Doi, K. Nozaki, Y. Takagi, and K.-i. Kikuta. 2010. Transdifferentiation of bone marrow-derived endothelial progenitor cells into the smooth muscle cell lineage mediated by tansforming growth factor-β1. Atherosclerosis. 211:114-121.
Lionetti, V., S. Cantoni, C. Cavallini, F. Bianchi, S. Valente, I. Frascari, E. Olivi, G. Aquaro, F. Bonavita, I. Scarlata, M. Maioli, V. Vaccari, R. Tassinari, A. Bartoli, F. Recchia, G. Pasquinelli, and C. Ventura. 2010. Hyaluronan Mixed Esters of Butyric and Retinoic Acid Affording Myocardial Survival and Repair without Stem Cell Transplantation. JBC, 285:9949-9961.
Hanley, E., J. Lauer, B. Micales, G. Lyons, and J. Shohet. 2008. Surface-directed differentiation of embryonic stem cells. Appl, Phys Lett, 92:193902.
Mattagajasingh, I., C.-S. Kim, A. Naqvi, T. Yamamori, T.A. Hoffman, S.-B. Jung, J. DeRicco, K. Kasuno, and K. Irani. 2007. SIRT1 promotes endothelium-dependent vascular relaxation by activating endothelial nitric oxide synthase. Proceedings of the National Academy of Sciences. 104:14855-14860.
Ohkawara, N., H. Ueda, S. Shinozaki, T. Kitajima, Y. Ito, H. Asaoka, A. Kawakami, E. Kaneko, and K. Shimokada. 2007. Hepatocyte Growth Factor Fusion Protein Having Collagen-Binding Activity (CBD-HGF) Accelerates Re-endothelialization and Intimal Hyperplasia in Balloon-injured Rat Carotid Artery. J Atherosclerosis and Thrombosis, 14:185-191.
Gousseva, N., K. Kugathasan, C.N. Chesterman, and L.M. Khachigian. 2001. Early growth response factor-1 mediates insulin-inducible vascular endothelial cell proliferation and regrowth after injury*. Journal of cellular biochemistry. 81:523-534.

For general cryopreservation of most primary cells. Contains FBS & DMSO. 040-50 по запросу

Cryopreserved RAOEC R304-05a по запросу

RAOEC Total Kit: Media, Subculture Reagents & Cells, Adult R304K-05a по запросу

Cultured and shipped at fourth passage in Rat Endothelial Cell Growth Medium. R305-25a по запросу
Proliferating RAOEC R305-75a по запросу
Proliferating RAOEC R305-6Wa по запросу
Proliferating RAOEC R305-96Wa по запросу

Total RNA prepared from Rat Aortic Endothelial Cells R304-R10a по запросу
Total RNA prepared from Rat Aortic Endothelial Cells R304-R25a по запросу

Added to Basal Medium to create Growth Medium R211-GS по запросу

100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 090K по запросу

Extended Family Products

AFS 123-100 по запросу

100 tests 028-S по запросу
500 tests 028-01 по запросу

25 x 24-Well Rxns TF101KS по запросу
250 x 24-Well Rxns TF101K по запросу

Related Products

Basal medium (contains no growth supplement).В  Add GS before use. R210-500 по запросу

All-in-one ready-to-use R211-500 по запросу

Basal medium & growth supplement sold together packaged separately R211K-500 по запросу
Growth medium kit without phenol red R211KPR-500 по запросу

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